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KMID : 1007520200290111587
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Food Science and Biotechnology
2020 Volume.29 No. 11 p.1587 ~ p.1595
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Simultaneous analysis of thirteen phytohormones in fruits and vegetables by SPE-HPLC?DAD
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Zhu Song
Chen Shang-Wei
Li Yue
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Abstract
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Determination of phytohormones have attracted increasing attentions in food safety field. In this study, an efficient and quantitative method was developed which can simultaneously determinate thirteen phytohormones in fruits and vegetables using solid phase extraction (SPE) combined with high performance liquid chromatography?diode array detection (HPLC?DAD). The samples were extracted with 80% methanol containing 0.5% (V/V) formic acid, and the extracts were then concentrated and purified using primary secondary amine (PSA) and C18 tandem dual SPE cartridges. The analytes were separated on a Waters XBridge™ C18 column and eluated utilizing a gradient elution program of water and methanol. Mean recoveries of the thirteen analytes varied from 74.69 to 92.40%, with relative standard deviations?
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KEYWORD
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Phytohormone, High performance liquid chromatography?diode array detection, Simultaneous analysis, Fruit and vegetable
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